تُجري وزارة الزراعة – المديرية العامة للزراعة وفقًا لأحكام قانون الشراء العام وبطريقة الظرف المختوم مناقصة عمومية لتلزيم تقديم كواشف مخبرية لزوم وزارة الزراعة – مديرية الثروة الحيوانية وفق دفتر الشروط هذا ومرفقاته التي تُعتبر كلها جزأً لا يتجزأ منه.يحق الاشتراك في هذه المناقصة للأشخاص الطبيعيين والمعنويين (شركات أو مؤسسات) الذين يتعاطون بيع وتوزيع الادوية والمواد البيطرية والمخبرية والمسجلين للعام 2025 في غرفة التجارة والصناعة والزراعة.يجري التلزيم بطريقة المناقصة العمومية على أساس تقديم سعر لكل مجموعة على حدة، ويحق للعارض ان يشترك في الصفقة على أساس مجموعة واحدة أو أكثر وفقاً للائحة الفنية المرفقة (الملحق رقم 1).يسند التلزيم مؤقتًا لكل مجموعة على حدة الى العارض المقبول عرضه شكلًا من الناحية الإدارية والفنية والذي قدم السعر الأدنى الاجمالي لكل مجموعة على حدة.المُلحق رقم (1)المواصفات الفنية الملحقة بدفتر الشروط الخاص لتلزيم تقديم كواشف مخبرية لزوم وزارة الزراعة – مديرية الثروة الحيوانية بطريقة المناقصة العموميةالمجموعة الأولى : ELISA KITSالبندرقم الصنفاسم الصنفالوصفالعددELISA KITS – HORSES1GlandersDouble antigen ELISA for the detection of antibodies directed against Burkholderia mallei in serum or plasma from horses, donkeys, mules. Method: Double Antigen ELISA Species: Equine and other susceptible species. For research use only on human samples. Specimens: Serum and plasma Coated antigen: Burkholderia mallei recombinant proteinConjugate: Purified Burkholderia mallei recombinant antigen-HRP conjugate (concentrated 10X)Plate Number: 5                    Reading: 450 or 405nm                           Positive and negative reference sera12African Horse SicknessIndirect ELISA for the detection of antibodies directed against the VP7 protein of the AHSV in serum or plasma from horses and donkeys: allowing for the detection of all the serotypes of the virus
Method: Indirect ELISA
Species: Horses, donkeys, mules. Contact us for use in other susceptible equids.
Specimens: Serum, plasma
Coated antigen: AHSV VP7 recombinant protein
Conjugate: Anti-equine IgG-HRP conjugate (concentrated 10X)
Plate Format: 12 x 8-well strips
Plate Number: 5                                 Reading: 450 or 405nm                        Positive and negative reference sera13West Nile Fever IgMIgM Antibody Capture ELISA kit for the detection of anti-prE IgM antibodies in horse serum and plasma.
Method: IgM Antibody Capture ELISA
Species: Equine
Specimens: Serum and plasma
Coated antibody: Anti-horse IgM polyclonal antibody
Conjugate: Anti-WNV prE HRP conjugate (10X)
Plate Number: 5
Plate Format: 12 x 8-well strips
Reading: 450 or 405nm
Positive and negative reference sera14EHV-1/EHV-4 Discrimination test IndirectIndirect Bidwell ELISA for the detection and discrimination of antibodies directed against equine herpesvirus types 1 and 4 in equine serum or plasma.
Method: Indirect ELISA
Species: Equine
Specimens: Serum and plasma
Coated antigen: EHV-1 or EHV-4 specific recombinant antigens
Conjugate: Anti-equine IgG-HRP conjugate (concentrated 10X)
Plate Number: 5
Plate Format: 12 x 8-well strips
Reading: 450 or 405nm
Positive and negative reference sera1ELISA KITS - RUMINANTS5FMD antigen DetectionIndirect FMDV Antigen detection ELISA and Serotyping: FMDV O, A, C, Asia1, SAT1-2
Method: Sandwich ELISA
Species: Multiple species, including ruminants, camels, horses, dogs and others. For research use only on human samples
Specimens: homogenates of epithelium vesicles and in vesicles fluid
Coated antibodies: anti-FMDV monoclonal antibodies (MAbs)
Conjugate: 
Microplates are supplied pre-coated with catching MAbs and with positive controls already incorporated onto plates.
Plate Number: ELISA kits are supplied with five ELISA plates. Up to ten samples can be tested in each plate.
Plate Format: 96 Wells
Reading: 45016Rift Valley Fever CompetitionCompetitive ELISA kit for the detection of anti-Rift Valley Fever (RVF) antibodies in serum or plasma from multiple species
Method: Competitive ELISA
Species: Multiple species, including ruminants, camels, horses, dogs and others. For research use only on human samples
Specimens: Serum or plasma
Coated antigen: RVF Virus nucleoprotein (NP)
Conjugate: Anti-NP-HRP conjugate (concentrated 10X)
Plate Number: 5
Plate Format: 12 x 8-well strips                Reading: 450 or 405nm        Positive and negative reference sera17Rift Valley Fever Ig M CaptureIgM Antibody Capture (MAC) ELISA for the detection of anti-nucleoprotein IgM antibodies in ruminant serum and plasma.
Method: IMAC (IgM Antibody Capture) ELISA
Species: Ruminants
Specimens: Serum or plasma
Coated antigen: RVF nucleoprotein (concentrated 10X)
Coated antibody: Anti-bovine-ovine-caprine IgM antibody
Conjugate: Anti-NP-HRP (concentré 10X)
Plate Number: 5
Plate Format: 6 x 16 well strips
Reading: 450 or 405nm  Positive and negative reference sera18Mycobacterium bovis complex / TuberculosisSandwich ELISA for the detection of ovine, bovine and caprine interferon gamma (IFN-g) in activated plasma or culture supernatant Method : Sandwich ELISA
Species : Bovine, ovine and caprine
Specimens : Activated plasma or culture supernatant
Coated antigen : Anti-ruminant IFN-g monoclonal antibody
Conjugate : Anti-ruminant IFN-g HRP Mab conjugate                        Plate Number: 2Plate Format: 12 x 8-well strips
Reading: 450 or 405nm  Positive and negative reference sera19PPRCompetitive ELISA kit for the detection of anti-PPRV nucleoprotein antibodies in sheep and goat serum or plasma
Method: Competitive ELISA
Species: Multiple species, including sheep, goats and others
Specimens: Serum or plasma
Coated antigen: PPRV nucleoprotein (NP)
Conjugate: Anti-NP-HRP conjugate (concentrated 10X)
Plate Number: 5
Plate Format: 12 x 8-well strips
Reading: 450 or 405nm Positive and negative reference sera110CapripoxDouble Antigen ELISA for the detection of antibodies against capripoxviruses including lumpy skin disease virus (LSDV), sheep pox virus (SPPV) and goat pox virus (GTPV) in serum or plasma from cattle, sheep, goats or other susceptible species. Method : Double Antigen ELISA
Species : Ruminants and other susceptible species
Specimens : Serum and plasma
Coated antigen : CPV purified antigen
Conjugate : CPV purified antigen-HRP (concentrated 10X)Plate Number: 5
Plate Format: 12 x 8-well strips Reading: 450 or 405nm Positive and negative reference sera111  Brucella Rose BengalRapid slide agglutination antigen for the detection of anti-Brucella abortus, melitensis, or suis antibodies in bovine, ovine, caprine, and porcine speciesFornmat : 10ml112BluetongueCompetitive ELISA kit for the detection of anti-VP7 antibodies in serum or plasma from multiple species. Detection of antibodies against all BTV serotypes,
Method: Competitive ELISA
Species: Multiple species, including sheep, goats, cattle, buffalo, deer, and others
Specimens: Serum or plasma
Coated antigen: Recombinant VP7
Conjugate: Anti-VP7-HRP conjugate (concentrated 10X)
Plate Number: 5                                               Plate Format: 12 x 8-well strips   Reading: 450 or 405nm                      Positive and negative reference sera1ELISA KITS _ POULTRY13Avian Influenza H9Indirect ELISA for the detection of antibodies against the hemagglutinin H9 of the Avian Influenza A virus in serum, plasma or egg yolk from chickens or turkeys
Method: Indirect ELISA
Species: Chickens and turkeys
Specimens: Serum, plasma or egg yolk
Coated antigen: Purified H9 recombinant protein
Conjugate: Anti-chicken-HRP conjugate (concentrated 10X)
Plate Number: 5
Plate Format: 12 x 8-well strips Reading: 450 or 405nm                        Positive and negative reference sera114Infectious Bronchitis Virus (IBV)Indirect ELISA for the detection of antibodies against IBV in chicken serum, plasma or egg yolk samples: detection of all existing variants
Method: Indirect ELISA
Species: Chickens (broilers, breeders and layers)
Specimens: Serum, plasma or egg yolk
Coated antigen: Purified IBV recombinant protein
Conjugate: Anti-chicken Ig-HRP conjugate (concentrated 10X)
Plate Number: 5                                         Plate Format: 12 x 8-well strips
Reading: 450 or 405nm                             Positive and negative reference sera115Infectious Laryngotracheitis VirusIndirect ELISA for the detection of antibodies directed against the avian Infectious Laryngotracheitis Virus (ILTV) in vaccinated and/or infected chicken sera
Method: Indirect ELISA
Species: Chickens (broilers, breeders and layers)
Specimens: Serum
Coated antigen: ILTV purified antigen
Conjugate: Anti-chicken-HRP conjugate (concentrated 10X)
Plate Number: 5                                           Plate Format: 12 x 8-well strips
Reading: 450 or 405nm                                  Positive and negative reference sera116Infectious Bursal Disease VirusIndirect ELISA for the detection of antibodies against the Infectious Bursal Disease Virus (IBDV) in chicken sera
Method: Indirect ELISA
Species: Chickens (broilers, breeders and layers)
Specimens: Serum or egg yolk
Coated antigen: IBD purified antigen
Conjugate: Anti-chicken-HRP conjugate (concentrated 10X)
Plate Number: 5                                   Plate Format: 12 x 8-well strips
Reading: 450 or 405nm                        Positive and negative reference sera117AdenovirusIndirect ELISA for the detection of  FADV Group 1 antibodies in chickens in chicken sera
Method: Indirect ELISA
Species: Chickens (broilers, breeders and layers)
Specimens: Serum or egg yolk
Plate Number: 5                                  Plate Format: 12 x 8-well strips
Reading: 450 or 405nm                    Positive and negative reference sera118Newcastle Disease Virus (NDV)Indirect ELISA for the detection of antibodies against the Newcastle Disease Virus (NDV) in chicken and turkey serum or egg yolk: detect infection or to quantify NDV antibody levels in birds vaccinated with conventional vaccines.
Method: Indirect ELISA
Species: Chickens (broilers, breeders, layers) and turkeys
Specimens: Serum or egg yolk
Coated antigen: NDV purified antigen
Conjugate: Anti-chicken-HRP conjugate (concentrated 10X)
Plate Number: 5
Plate Format: 12 x 8-well strips
Reading: 450 or 405nm
Positive and negative reference sera1ELISA KITS _ PET19RabiesELISA kit for detection and titration of IgG anti rabies virus glycoprotein in dogs, cats and foxes serum.                        Method: Indirect ELISA
Species: dogs, cats and foxes
Specimens: Serum
Coated antigen: rabies virus glycoprotein
Plate Format: 12 x 8-well strips
Reading: 450 or 405nm   4 EU/ml Positive control 0.5 EU/ml positive control1المجموعة الثانية : PCR KITSرقم الصنفاسم الصنفالوصفالعدد20AVIAN INFLUENZA KITOne-step, real-time, RT-PCR amplification of Avian Influenza Virus matrix gene RNA and RNA Internal Positive Control it includes
• RT-PCR buffer, 
• RT-PCR Enzyme Mix, 
• AIV-Matrix TaqMan™ Primer Probe mix: Reporter dye : FAM
• Detection enhancer
• Nuclease-free water
• Positive Control
• RNA Internal Positive Control: Reporter dye :Vic 
• Reactions: 100121Avian Influenza H9One-step, real-time, RT-PCR amplification of Avian Influenza Virus H9 RNA and RNA Internal Positive Control it includes
• RT-PCR buffer, 
• RT-PCR Enzyme Mix, 
• AIV-H9 TaqMan™ Primer Probe mix: Reporter dye : FAM
• Detection enhancer
• Nuclease-free water• Positive Control
• RNA Internal Positive Control: Reporter dye :Vic 
• Reactions: 100122Mycobacterium tuberculosis complex DuplexReal-time qPCR for the qualitative detection of a sequence in the genome of members of the Mycobacterium tuberculosis complex (MTC) in lymph nodes and peripheral tissues.
Method: Real-time qPCR - Duplex - Qualitative
Species: Cattle, sheep, goats
Sample types: Lymph nodes and peripheral tissues
Exogenous internal control to be extracted along with field samples
Reactions: 100123One Step RT-PCRPCR Mix: 
One Step RT-PCR Enzyme Mix
One Step RT-PCR Buffer
dNTP Mix (10 mM each)
RNase-Free Water1المجموعة الثالثة : ELECTOPHORESISرقم الصنفاسم الصنفالوصفالعدد2410x TBE electrophoresis BufferFormat: 1 liter125 DNA Ladder 100pb100 bp DNA Ladder: for sizing and approximate quantification of a double-stranded DNA in the range of 100 bp to 1,000 bp on agarose or polyacrylamide gels. 
The DNA ladder consists of 10 DNA fragments and is provided with 6X TriTrack DNA Loading Dye126DNA Ladder 25 to 700 bpLow Range DNA Ladder: for sizing and approximate quantification of a double-stranded DNA in the range of 25 bp to 700 bp on agarose or polyacrylamide gels. 
The DNA ladder consists of 10 DNA fragments and is provided with 6X TriTrack DNA Loading Dye127 DNA Ladder 50 bp50 bp DNA Ladder: for sizing and approximate quantification of a double-stranded DNA in the range of 50 bp to 1,000 bp on agarose or polyacrylamide gels. 
The DNA ladder consists of 13 DNA fragments and is provided with 6X TriTrack DNA Loading Dye128DNA Ladder 100 bp Plus100 bp Plus DNA Ladder: for sizing and approximate quantification of a double-stranded DNA in the range of 100 bp to 3,000 bp on agarose or polyacrylamide gels. 
The DNA ladder consists of 14 DNA fragments and is provided with 6X TriTrack DNA Loading Dye.1